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Ferritin from germinated soybean seeds was purified by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, gel filtration chromatography on Sephacryl S-300, and HPLC with Bio-Scale Q2 column. SDS-PAGE analysis showed that the purified ferritin is composed of subunit with an apparent M, 21,000. The molecular mass of the native soybean ferritin estimated by gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be 510∼560 kDa. Soybean ferritin contained 833 ㏖ Fe/㏖ protein, which is 31-fold more iron than pumpkin ferritin and stained positive for iron on nondenaturing gel. Soybean ferritin cross-reacted with anti-soybean rabbit ferritin antiserum.
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